NA2000
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Useful Links & Tools
NA2000
Sigma
GenElute™ Blood Genomic DNA Kit
sufficient for 10 purifications
Description
| Other Notes | Sigma's GenElute Blood Genomic DNA Kit provides a simple and convenient technique to isolate high quality genomic DNA from fresh or aged (older than 24 hours) whole blood. This kit combines the advantages of a silica-based system with a microspin format, eliminating the need for expensive resins and hazardous organic compounds. |
| For additional information, please see www.sigma-aldrich.com/genomicdna. | |
| Features and Benefits | • Typical yields 4-10 μg from 200 μl of whole blood • Isolate high quality genomic DNA from fresh or aged blood • Compatible with many anticoagulants, including EDTA, Heparin and Sodium Citrate • Less than 40 minutes from whole blood to isolated genomic DNA • A prewash solution is provided to ensure the removal of all contaminants associated with older blood samples • Purity of genomic DNA has an A260/A280 ratio between 1.6 and 1.9 • An RNase solution is provided for added convenience • Isolate DNA up to 50 kb in length • Resulting DNA can be used directly in PCR, restriction digestion, and Southern blots |
| Principle | Whole blood is digested with proteinase K and is lysed following the addition of a chaotropic salt-containing solution. DNA is bound to the silica-based membrane and the remaining lysate is removed by centrifugation. A wash step removes contaminants associated with aged whole blood samples and the DNA is eluted with buffer into a collection tube. Purified DNA is ready for downstream applications such as restriction endonuclease digestions (Fig. 1), PCR (Fig. 3), Southern blots and sequencing reactions. |
| Legal Information | GenElute is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| usage | sufficient for 10 purifications |
Safety
| Hazard Codes | Xn |
| Risk Statements | 20/21/22-36/37/38-42 |
| Safety Statements | 22-26-36 |
